Metabolomics and proteomics identify the toxic form and the associated cellular binding targets of the anti-proliferative drug AICAR
Metabolomics and proteomics identify the toxic form and the associated cellular binding targets of the anti-proliferative drug AICAR The antibody against the α1AMPK subunit used for western blot analysis and the antibody against the α2AMPK isoform, which were kind gifts of G. Endogenous AMPK was immunoprecipitated using 500 μg protein from cell lysates by incubation…
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